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Effect of GIP on cAMP accumulation of in fat cells. Following a 10-min incubation under different conditions, cAMP extracted from both rat fat and differentiated 3T3-L1 cells was measured by radioimmunoassay. Basal signifies cAMP levels in the untreated sample sets. 1 μM isoproterenol (Iso) and 20 μM forskolin (Forsk) were used as positive controls. Similar results were obtained from at least two independent experiments. Data are expressed as the mean ±SE in pmol/mL.
