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. Author manuscript; available in PMC: 2008 Jan 9.
Published in final edited form as: Gastroenterology. 2007 Sep 14;133(6):1796–1805. doi: 10.1053/j.gastro.2007.09.005

Figure 6.

Figure 6

Figure 6

Effects of GIP and insulin on the accumulation of GLUT-4 in the plasma membrane. (A) Differentiated 3T3-L1 adipocytes were incubated in the presence of either 20 nM GIP or 10 nM insulin with or without the GIP-specific antagonist ANTGIP (AG) for 1 h. Membrane fractions were extracted and probed for the accumulation of GLUT-4 protein. (B) 3T3-L1 preadipocytes were grown in chamber slides and differentiated, and probed for the accumulation of GLUT-4 protein. Cells were visualized using confocal laser scanning microscopy. Representative images are shown, and similar images were visualized in three additional independent experiments. (C) Differentiated 3T3-L1 adipocytes were pre-treated with 1 μM wortmannin for 30 min prior to additional incubation in the presence or absence of GIP. Fat cells were stained with polyclonal GLUT-4 primary and counterstained with FITC conjugated secondary antibodies.