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. 2000 Jul 10;150(1):243–252. doi: 10.1083/jcb.150.1.243

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Colocalization of ICAM-5 at cell–cell contact sites in Paju neuronal cells. Human ICAM-5 transfected into Paju cells was expressed at cell–cell contact sites and the uropods of Paju-ICAM-5 cells (a and b), but not in Paju-WT/Paju-ICAM-5 contact regions (c and d). When Paju-ICAM-5 cells were cultured in the presence of the antiadhesive-ICAM-5 mAbs, like TL-3 (i and j), the localization of ICAM-5 at contact sites was diminished as compared with treatment with control mAb (antiglycophorin A; e and f) or the mAb TL-1 treatment (g and h), which does not affect adhesion. a, c, e, g, and i, Phase-contrast photographs; b, d, f, h, and j, immunofluorographs. Bars, 20 μm.