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. 2000 Jul 10;150(1):243–252. doi: 10.1083/jcb.150.1.243

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Inhibition of dendritic outgrowth from hippocampal neurons by anti–ICAM-5 antiserum or soluble ICAM-5 D1–2 or ICAM-1. a, Rat E19 hippocampal neurons were cultured on ICAM-5 D1–2 coated surfaces for 48 h. The neurons were stained by anti–MAP-2 (red) and anti–ICAM-5 (green). The polyclonal antibody against rat ICAM-5, 1000J (100 μg/ml), significantly inhibited dendrite outgrowth, compared with the control polyclonal serum (ctrl pAb). A similar inhibitory effect was seen with soluble ICAM-5 D1–2 (100 μg/ml), but not with ICAM-1. Arrows indicate neurites that may be axons stained by anti–MAP-2, but not by anti–ICAM-5. Bars, 20 μm. In b, the number of dendrite-bearing hippocampal neurons subjected to different treatments was counted. 100 neurons in each case were examined.

Inhibition of dendritic outgrowth from hippocampal neurons by anti–ICAM-5 antiserum or soluble ICAM-5 D1–2 or ICAM-1. a, Rat E19 hippocampal neurons were cultured on ICAM-5 D1–2 coated surfaces for 48 h. The neurons were stained by anti–MAP-2 (red) and anti–ICAM-5 (green). The polyclonal antibody against rat ICAM-5, 1000J (100 μg/ml), significantly inhibited dendrite outgrowth, compared with the control polyclonal serum (ctrl pAb). A similar inhibitory effect was seen with soluble ICAM-5 D1–2 (100 μg/ml), but not with ICAM-1. Arrows indicate neurites that may be axons stained by anti–MAP-2, but not by anti–ICAM-5. Bars, 20 μm. In b, the number of dendrite-bearing hippocampal neurons subjected to different treatments was counted. 100 neurons in each case were examined.