Table 1.

Quantification of the Ezrin Content in the Different Pools

	Cell debris	Cytosol	Membrane	Membrane ins.
	%	%	%	%
Total	8.8	81.9	9.3	0.057
Monomers	–	69.6	6.7	–
Oligomers	–	12.3	2.6	–

The amount of ezrin was quantified by Western blotting serially diluted fractions. Cells were mechanically cracked. Cell debris refers to the 600-g pellet that also contained nuclei and unbroken cells. The supernatant was then separated by a 100,000-g centrifugation into a cytosolic supernatant and a membrane-containing pellet. The pellet was extracted with a high salt Triton X-100 buffer. Membrane ins. refers to the 100,000-g pellet after its extraction (see Materials and Methods). Monomers and oligomers were separated by gel filtration chromatography and the corresponding fractions were pooled. These data are the means of two independent fractionations.