Table 2.
Summary of per Mutant Characterization
| Allele | Gene | Temperature conditional phenotype | Interaction with Δire | Protein processing defect | Suppressing by Hac1ip |
|---|---|---|---|---|---|
| per1-1 | ts | SN | Gas1p | No | |
| per1-2 | SN | Yes | |||
| per2-1 | MCD4 | ts | SL | Gas1p | Yes |
| per3-1 | SL | Glycosylation | Yes | ||
| per4-1 | LHS1 | SL | Yes | ||
| per4-2 | LHS1 | cs | SL | Yes | |
| per5-1 | RFT1 | SL | Glycosylation | Yes | |
| per6-1 | SL | Glycosylation | Yes | ||
| per6-2 | ts | SL | Glycosylation | Yes | |
| per7-1 | SL | CPY*HA degradation | Yes | ||
| per8-1 | SON1 | cs | SL | CPY*HA degradation | Yes |
| per9-1 | SL | Gas1p/CPY*HA degradation | Yes | ||
| per10-1 | SL | Yes | |||
| per10-2 | SL | Yes | |||
| per11-1 | SN | Yes | |||
| per12-1 | ts | SL | Glycosylation | Yes | |
| per13-1 | GPI10 | ts | SN | Gas1p/CPY*HA degradation | Yes |
| per14-1 | SL | Glycosylation | Yes | ||
| per15-1 | ts/cs | SL | Gas1p | No | |
| per16-1 | UBC7 | SN | CPY*HA degradation | Yes |
per mutant allele designations listed with corresponding gene names, if known. ts, Temperature-sensitive: impaired or no growth at 37 °C; cs, cold-sensitive: impaired or no growth at 16°C; SL, synthetic lethal: mutation is lethal in combination with Δire1, as determined by tetrad analysis; SN, synthetically negative: mutation causes severely impaired growth in combination with Δire1, as determined by tetrad analysis.