Table 1.

Quantitation of Cytochrome c and Mitotracker Staining in Sympathetic Neurons Treated with Various Conditions

	Percent total cytochrome c +	Percent total Mitotracker+
+NGF (untreated)	100	100
+CCCP 10 μM 100 μM	100 100	98 ± 2 0
+Valinomycin 10 μM 100 μM	100 100	0 0
+KCl 100 mM	100	100
+Ouabain 10 μM 100 μM	100 100	100 99 ± 1

The number of cells showing intact mitochondrial cytochrome c and Mitotracker staining after treatment with various condition (Fig. 1) is shown as a percentage of total number of cells examined. Cells were considered to be negative for cytochrome c or Mitotracker only if the characteristic punctate staining pattern was lost completely. Results are mean ± range of two independent experiments with 100–200 cells counted for each condition.