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EGFP-NF-M accumulations begin to develop in proximal neurites of some transfected neurons treated with 100 μM glutamate for 180 min (i.e., 320 min after transfection). Images shown are of EGFP-NF-M in proximal regions of neurites 320 min after transfection in untreated cells (a–c) and cells treated with 100 μM glutamate for 180 min and in which neurofilament accumulations are starting to develop (d–f). Bar, 25 μm.
