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. 2000 Jul 10;150(1):165–176. doi: 10.1083/jcb.150.1.165

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Glutamate activates p42 and p44 MAPKs and does not alter the levels of neurofilament or tubulin proteins in cortical neurons. 7-d-old cortical neurons were either untreated (un), or treated with 100 μM glutamate for 30, 60, or 120 min, and then analyzed by 12% SDS-PAGE and immunoblotting. No change in the total levels of either p42 or p44 MAPK (arrowheads), but a noticeable increase in the active forms of both these kinases was observed after glutamate treatment. Glutamate did not alter the levels of NF-L, NF-M, NF-H, or tubulin. An identically loaded Coomassie-stained gel is shown at the bottom to demonstrate equal protein loading of the samples.