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Glutamate induces increased phosphorylation of NF-M/NF-H side-arms in cell bodies of EGFP-NF-M–transfected neurons. Cells were transfected with EGFP-NF-M and, 320 min after transfection, were fixed and immunostained with SMI36. a and b are untreated; c and d are treated with 100 μM glutamate for 180 min. a and c show EGFP-NF-M; b and d show SMI36 labeling. Note the proximal accumulation of neurofilament protein in the glutamate-treated cell. Bar, 25 μm.
