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. 2000 Jul 10;150(1):1–12. doi: 10.1083/jcb.150.1.1

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The CENP-meta locus in D. melanogaster. (A) Sequence analysis of 24 kb of the 32D-E region of chromosome 2 revealed the presence of five genes encoding two new kinesin related proteins, CENP-meta and CENP-ana, a putative palmitoyl protein thioesterase (PPT-2), an exo/endonuclease (MRE 11), and the 3′ untranslated region of the abrupt gene. Exon structure, sites of P-element insertions, and direction of transcription (arrows) are shown as well as a schematic representation of both the CENP-meta cDNA and encoded protein. In addition, the location of the probe used for RNA blot analysis, the location of the peptide used to generate an antibody to CENP-meta, a protein expression construct used for antibody purification, and a subset of cDNA clones isolated for CENP-meta are indicated. The deduced amino acid sequence of CENP-meta is available from EMBL/GenBank/BDGP accession number AF220353. (B) Domain comparisons of CENP-meta, CENP-ana, HCENP-E and XCENP-E. Length in amino acids is indicated for each domain. (C) Sequence comparison indicating the percentage of identity between species for each domain. Human CENP-E (HCENP-E); Xenopus CENP-E (XCENP-E).