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. 2000 Jul 10;150(1):27–40. doi: 10.1083/jcb.150.1.27

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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In situ hybridization analysis of gene expression in day 10 chick embryo fibula. Longitudinal sections of fibula and surrounding tissues from C-1-1 virus-infected embryos were processed for in situ hybridization with indicated probes. (A) Phase micrograph; (B) type II collagen hybridization, showing a characteristic strong signal in epiphysis and metaphysis and a much reduced signal in hypertrophic diaphysis; (C) type X collagen hybridization, showing strong signal in hypertrophic diaphysis; and (D) hybridization with common ch-ERG/C-1-1 probe, showing lack of signal in fibula but extremely strong signal in surrounding perichondrial and connective tissues. Bar, 200 μm.