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. 2000 Jul 10;150(1):89–104. doi: 10.1083/jcb.150.1.89

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Ability of selected single and double point mutants to complement growth of mrs6Δ null strain. A, Indicated mutants were expressed in the mrs6Δ null strain using the single copy CEN plasmid as described in Materials and Methods. B, Indicated mutants were expressed using the single copy CEN plasmid, or multicopy 2μ plasmid in wild-type cells. The ability of overexpressed Mrs6p double mutants to partially inhibit growth is illustrated by reduced level of colonies appearing on the plate. C, Indicated mutants were expressed in the mrs6Δ null strain using the single copy CEN plasmid as described in Materials and Methods. 5,000, 2,000, 500, and 50 cells were spotted onto a plate. The growth was assessed after a 2–4-d incubation at 30°C.

Ability of selected single and double point mutants to complement growth of mrs6Δ null strain. A, Indicated mutants were expressed in the mrs6Δ null strain using the single copy CEN plasmid as described in Materials and Methods. B, Indicated mutants were expressed using the single copy CEN plasmid, or multicopy 2μ plasmid in wild-type cells. The ability of overexpressed Mrs6p double mutants to partially inhibit growth is illustrated by reduced level of colonies appearing on the plate. C, Indicated mutants were expressed in the mrs6Δ null strain using the single copy CEN plasmid as described in Materials and Methods. 5,000, 2,000, 500, and 50 cells were spotted onto a plate. The growth was assessed after a 2–4-d incubation at 30°C.