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. 2000 Jul 10;150(1):89–104. doi: 10.1083/jcb.150.1.89

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Fluorescence assay to measure Mrs6p-Rab3A interactions in vitro. A and B, Effect of Mrs6p wild-type and mutants on the intrinsic dissociation of mant-GDP from Rab3A. Mrs6p was added at the indicated final concentration (μM). The interaction of Mrs6p with prenylated (A) and unprenylated (B) Rab3A was determined by measuring the decrease in relative fluorescence (λ excitation 360 nm, λ emission 440 nm) that accompanies the release of mant-GDP from Rab3A as described in Materials and Methods. C, The interaction of Mrs6p mutants with Rab3A was determined by mixing 100 nM Rab3A with 2.5 μM Mrs6p wild-type or mutant protein as indicated.