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. 2000 Jul 10;150(1):89–104. doi: 10.1083/jcb.150.1.89

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Distribution of wild-type and mutant Rab proteins between cytosol and membranes. Wild-type and clone 14 mrs6 ^ts strain were incubated at the indicated temperature. After 5 h, the same OD of cells was lysed and subjected to centrifugation at 100,000 g to generate a P100 membrane fraction (p) and an S100 cytosol fraction (s). The amount of Ypt1p in each fraction was quantitated by immunoblotting with specific antibody. The inset illustrates that the amount of total Rab at the 5-h time point that was detected using immunoblotting was the same in both the wild-type and ts strains at either 30°C or 37°C.