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. 2000 Jul 10;150(1):89–104. doi: 10.1083/jcb.150.1.89

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Analysis of prenylation activity of Mrs6p single and double mutants in vitro. A, Single and double mutants that partially or fully complemented growth were expressed in the mrs6Δ strain and extracts prepared for assay in the presence or absence of recombinant Ypt1p as described in Materials and Materials. B, To assess the prenylation activity of inviable double mutants, these were expressed in the mrs6 ^ts strain and extracts prepared for assay as indicated. The assay profile of clone 14 is typical for that observed for clones 13, 17, and 38. Double mutants were assayed in the clone 14 strain at 37°C.