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. 2000 Jul 10;150(1):89–104. doi: 10.1083/jcb.150.1.89

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Analysis of vesicle-mediated trafficking of mrs6 single mutants. The indicated strains in mrs6Δ null cells were prepared and the cultures were labeled with [³⁵S]methionine/cysteine for 20 min, then chased for 5 or 30 min. CPY was immunoprecipitated from extracts derived from each culture. The positions of p1-CPY (ER), p2-CPY (Golgi), and mature (m) CPY (vacuole) are indicated to the left. In wild-type cells incubated at 37°C transport is very rapid. Here, CPY is largely recovered in the mature form, even after a 5-min chase period. B, The distribution of CPY in the p1, p2, and m forms at the 5-min (top) and 30-min (bottom) time points for each mutant were quantitated using densitometry.