Table 1.

Effect of Single Point Mutations in Mrs6p on Growth of the 6Δ Null Strain

Region	Location	Residue mutated	Growth at 30°C	Yeast GDI residue	Mammalian GDI residue
Wild-type	−	−	+++	−	−
SCR1A	a1 strand	D48N	+++	11	5
SCR1A	a1 loop A	G53A	+++	16	11
SCR1A	a1 loop A	G55A	+++	18	13
SCR1B	a2 loop f1	D76N	++	39	34
SCR1B	a2 loop f1	Y81V	+++	44	39
−	B helix	K99D	+++	59	55
SCR2	f2 strand	R127A	+++	78	70
SCR2	b2 strand	E174Y	+++	125	117
SCR2	D helix	T185D	+++	136	128
SCR2	E helix	R195A	+	146	138
SCR2	E helix	K199D	++	150	142
SCR2	E loop F	E208Q	+++	160	152
−	F helix	L226Y	+++	180	172
SCR3A	H loop e3	D269A	+++	225	217
SCR3A	H loop e3	V270R	+++	226	218
SCR3A	H loop e3	Y271F	+++	227	219
SCR3B	I helix	E286S	+	241	233
SCR3B	I helix	Q289L	+++	244	236
SCR3B	I helix	R293A	+	248	240
SCR3B	a3 strand	T301P	+++	256	248

−, Dead; +, slow; ++, slower than wild-type; +++, like wild-type.