Figure 1.

Dynamin-2 specifically induces cell toxicity in tTA HeLa cells. (A) Phase contrast micrographs of tTA-HeLa cells infected with an equivalent moi of adenovirus expressing either dyn1^wt, dyn2^wt, or dyn2^K44A under control of a tetracycline-regulatable promoter, as described in Materials and Methods. After infection, cells were incubated for 36 h in media containing the indicated concentration of tetracycline. (B) Western blot analysis using the pan-dynamin antibody 748 showing endogenous dynamin-2 (detected in control lanes and in lanes where exogenous expression is suppressed in the presence of 1 μg/ml tet) and tetracycline-dependent expression levels of exogenous dyn1^wt, dyn2^wt, or dyn2^K44A. (C–E) Immunofluorescence images of dynamin staining in uninfected tTA HeLa cells (C) or tTA HeLa cells infected with adenovirus encoding dyn1^wt (D) or dyn2^wt (E) and cultured for 24 h in the absence of tetracycline. Bar, 20 μm.