Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Jul 10;150(1):119–130. doi: 10.1083/jcb.150.1.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

SDS-PAGE of 26S proteasomes. Fractions 10 and 13–20 from the sucrose density gradient centrifugation were separated on a 12.5% SDS gel and the proteins were stained with Coomassie blue. Molecular weights of the marker proteins are shown on the left. For assignment of bands see Fig. 4. Fraction 10 (∼22% sucrose) contained pure 20S proteasomes, and fractions 13–20 (∼24–28%) displayed the typical pattern of subunit components exhibited by 26S proteasomes. Fractions 13–15 were contaminated with another protein complex (possibly a homologue of GroEL).