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Effects of ACAPs are dependent on both the formation of Arf-GTP and GAP activity. Cells were transfected with either plasmids directing expression of the dominant-negative (T27N) form or the constitutively active (Q67L) form of Arf6 and ACAP1. Cells expressing Arf6 and [R448Q]ACAP1, which lacks GAP activity, were treated with AlF4 for 30 min before preparation for immunofluorescence. Bars, 10 μm.
