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. 2000 Oct 30;151(3):627–638. doi: 10.1083/jcb.151.3.627

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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PIP2 and PA dependence of ACAP/ASAP family Arf GAPs. Arf6 was used as a substrate for ACAP1 and ACAP2. Arf1 was used as a substrate for 0.2 μg/ml ASAP1 purified from bovine brain. PIP2 concentrations are indicated. The reactions contained 380 μM PA, where indicated. The rates are given in hydrolyses/min.