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. 2000 Oct 30;151(3):697–708. doi: 10.1083/jcb.151.3.697

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The mitotic long MLCK displays decreased kinase activity towards the RLC. (A) The long MLCK was immunoprecipitated with the MLCK mAb and the immune complexes reacted with affinity-purified pAbs to MLCK. The bands at the bottom of the blot are the IgG heavy chains that cross-react with the secondary antibody used for chemiluminescent detection. (B) Immunoblot analysis confirmed that equivalent amounts of the long MLCK were present in the immunoprecipitates. (C) Symbols indicate the following: □, activity of the long MLCK from interphase extracts; ▪, activity of the long MLCK from interphase extracts treated with alkaline phosphatase; ○, activity of the long MLCK from mitotic extracts; and •, activity of the long MLCK from mitotic extracts treated with alkaline phosphatase. Values represent the mean and the SEM for three (with alkaline phosphatase) or eight (without alkaline phosphatase) independent experiments.