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Distribution of truncated MLCKs during interphase. Fluorescence micrographs are of HeLa cells transfected with the NH2-terminal extension, NH2-terminal extension containing all five DXRXXL motifs, and the avian short MLCK-GFP containing all five DXRXXL motifs. The top row shows representative images of intact cells. The bottom row shows representative images of Triton X-100–permeabilized cells. Bar, 10 μm.
