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. 2000 Oct 30;151(3):539–550. doi: 10.1083/jcb.151.3.539

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB0001040.f3ab.jpg — HRP-stimulated EGF-dependent endocytosis requires the small GTPase Rab5. (A) Cells expressing EGFR:WT were either transfected with virus alone (control: ○, •), virus encoding Rab5 (▴, ▵), or virus encoding Rab5:N34 (□, ▪). After transient transfection, the amount of HRP internalized at 37°C for the indicated times was quantified in the presence (▵, ○, □) or absence (•, ▴, ▪) of EGF. This experiment was repeated at least three times with similar results. (B) Cells expressing EGFR were transfected with virus encoding Rab5, Rab5:N34, Rab11, Rab11:mB1, Rab7, or Rab7:N22. After transfection, the internalization of HRP (37°C for 10 min) was quantified in the presence or absence of 100 nM EGF. Values are mean ± SD, n = 3. (C) Cells expressing EGFR:WT were either transfected with virus alone, virus encoding Rab5, virus encoding Rab5:N34, virus encoding Rab5:N34/ΔN19, virus encoding Rab5:ΔN19, virus encoding Rab5:ΔC4, or virus encoding Rab5:ΔC4/N34. After transient transfection, the amount of HRP internalized at 37°C for 15 min was quantified in the presence or absence of 100 nM EGF. Values are mean ± SD, n = 3.

graphic file with name JCB0001040.f3c.jpg — HRP-stimulated EGF-dependent endocytosis requires the small GTPase Rab5. (A) Cells expressing EGFR:WT were either transfected with virus alone (control: ○, •), virus encoding Rab5 (▴, ▵), or virus encoding Rab5:N34 (□, ▪). After transient transfection, the amount of HRP internalized at 37°C for the indicated times was quantified in the presence (▵, ○, □) or absence (•, ▴, ▪) of EGF. This experiment was repeated at least three times with similar results. (B) Cells expressing EGFR were transfected with virus encoding Rab5, Rab5:N34, Rab11, Rab11:mB1, Rab7, or Rab7:N22. After transfection, the internalization of HRP (37°C for 10 min) was quantified in the presence or absence of 100 nM EGF. Values are mean ± SD, n = 3. (C) Cells expressing EGFR:WT were either transfected with virus alone, virus encoding Rab5, virus encoding Rab5:N34, virus encoding Rab5:N34/ΔN19, virus encoding Rab5:ΔN19, virus encoding Rab5:ΔC4, or virus encoding Rab5:ΔC4/N34. After transient transfection, the amount of HRP internalized at 37°C for 15 min was quantified in the presence or absence of 100 nM EGF. Values are mean ± SD, n = 3.