Figure 6.

Formation of enlarged Rab5-positive endosomes requires an intact cytoplasmic domain of the EGFR. Cells expressing either EGFR:WT, EGFR:c'973, EGFR:c'991, or EGFR:c'1000 were transfected with virus encoding GFP-Rab5a. After transient transfection, the cells were incubated with EGF and the formation of enlarged endosomes were visualized by confocal microscopy as described in Materials and Methods. This experiment was repeated three times and the results were highly reproducible.