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. 2000 Oct 30;151(3):483–494. doi: 10.1083/jcb.151.3.483

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB0006120.f4ab.jpg — Inhibition of apoptosis by constitutively active Akt and Bcl-2. (A) Fluorescence photomicrographs of Hoechst 33342 staining of HMN1 cells transfected with pCMV, human Bcl-2, pCR3.1, or myr-AktΔ4-129. Cells were treated with 30 μM C2-ceramide for 5 h. (B) Constitutively active Akt and Bcl-2 inhibit ceramide-induced HMN1 cell apoptosis. Cells were treated with 30 μM C2-ceramide for 5 h and apoptosis was assayed by Hoechst 33342 staining. Data represent mean ± SEM for three independent experiments. (C) Constitutively active Akt and Bcl-2 inhibit DNA fragmentation in HMN1 cells. Cells were treated with 30 μM C2-ceramide for 0, 3, or 5 h, and soluble DNA was extracted for DNA fragmentation assay. Data are from a representative experiment performed three times.

graphic file with name JCB0006120.f4c.jpg — Inhibition of apoptosis by constitutively active Akt and Bcl-2. (A) Fluorescence photomicrographs of Hoechst 33342 staining of HMN1 cells transfected with pCMV, human Bcl-2, pCR3.1, or myr-AktΔ4-129. Cells were treated with 30 μM C2-ceramide for 5 h. (B) Constitutively active Akt and Bcl-2 inhibit ceramide-induced HMN1 cell apoptosis. Cells were treated with 30 μM C2-ceramide for 5 h and apoptosis was assayed by Hoechst 33342 staining. Data represent mean ± SEM for three independent experiments. (C) Constitutively active Akt and Bcl-2 inhibit DNA fragmentation in HMN1 cells. Cells were treated with 30 μM C2-ceramide for 0, 3, or 5 h, and soluble DNA was extracted for DNA fragmentation assay. Data are from a representative experiment performed three times.