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. 2000 Oct 30;151(3):685–696. doi: 10.1083/jcb.151.3.685

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB0005023.f7a.jpg — Calpain inhibitors and dominant-negative μ-calpain prevent formation of integrin-containing clusters. (A) BAE cells were detached and replated on fibronectin-coated coverslips in the presence of calpeptin (100 μg/ml) or MDL (250 μM) for 1 h, fixed, and permeabilized. Cells were stained with antibodies against β3 integrin, actin filaments were detected with TRITC-labeled phalloidin. (B) BAE cells were grown to 80% confluency and transiently transfected with plasmid encoding HA-tagged dominant-negative μ-calpain. Cells were detached and replated on fibronectin-coated coverslips for 1–3 h, fixed, and permeabilized. Cells were stained with antibodies against vinculin. Transfected cells were detected with HA-myc antibody and actin filaments were detected with TRITC-labeled phalloidin. Two independent fields are shown. Bar, 20 μm.

graphic file with name JCB0005023.f7b.jpg — Calpain inhibitors and dominant-negative μ-calpain prevent formation of integrin-containing clusters. (A) BAE cells were detached and replated on fibronectin-coated coverslips in the presence of calpeptin (100 μg/ml) or MDL (250 μM) for 1 h, fixed, and permeabilized. Cells were stained with antibodies against β3 integrin, actin filaments were detected with TRITC-labeled phalloidin. (B) BAE cells were grown to 80% confluency and transiently transfected with plasmid encoding HA-tagged dominant-negative μ-calpain. Cells were detached and replated on fibronectin-coated coverslips for 1–3 h, fixed, and permeabilized. Cells were stained with antibodies against vinculin. Transfected cells were detected with HA-myc antibody and actin filaments were detected with TRITC-labeled phalloidin. Two independent fields are shown. Bar, 20 μm.