Figure 2.
Analysis of human COX-2 mRNA in cultured cells (a and b) by Northern blotting and murine COX-2 mRNA (c) by RT-PCR. a shows the effect of sodium salicylate at concentrations up to 10−5 M on HUVEC COX-2 mRNA induced by PMA. Concurrent glyceraldehyde 3-phosphate dehydroxygenase (GAPDH) mRNA blotting was included as the control. b shows the effect of aspirin on HFF COX-2 mRNA induced by PMA. c shows the in vivo effects of aspirin at dosages of 10 and 30 mg/kg on murine macrophage COX-2 mRNA expression induced by LPS. Lanes: 1–3, LPS-treated mice in the absence (lane 1) and presence (lanes 2 and 3) of aspirin; 4, normal mouse peritoneal macrophages. Glyceraldehyde 3-phosphate dehydroxygenase (GAPDH) mRNAs, which were included to serve as internal controls, are shown in the lower panel.