Skip to main content
. 1999 Apr 27;96(9):5292–5297. doi: 10.1073/pnas.96.9.5292

Figure 4.

Figure 4

(ad) Western blot analysis of COX-2 proteins in cultured HFF. (a and b) The effect of sodium salicylate on PMA-induced COX-2 proteins with (a) and without (b) FBS in the medium. (c and d) The effects of indomethacin (c) and NS398 (d) on COX-2 proteins in HFF. Std., purified COX-2 proteins as standards. (ef) COX-2 activity in cultured HFF. (e) The effect of sodium salicylate (10−5 M) on basal and PMA-induced PGE2 levels. Each bar represents mean ± SD of four determinations. (f) The effect of sodium salicylate (10−5 M) on eicosanoid production in response to [1-14C] arachidonate treatment in HFF stimulated with PMA. The data are representative of two experiments with similar results. ♦ denotes PMA plus vehicle, ● denotes PMA plus sodium salicylate, and ▴ denotes PMA plus NS398.