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. Author manuscript; available in PMC: 2008 Jan 9.
Published in final edited form as: Cell Microbiol. 2006 Jul 18;8(12):1932–1945. doi: 10.1111/j.1462-5822.2006.00759.x

Fig. 3. Cryptosporidium parvum infection induces activation and membrane translocation of ASM in infected cells.

Fig. 3

A. A representative flow cytometry plot of ASM labelling in H69 cells. Right shift of fluorescence to ASM was found in non-permeabilized cells after exposure to C. parvum. No change of fluorescence was found in membrane-permeabilized cells.

B. Quantitative analysis of fluorescent intensity to ASM by flow cytometry from three independent experiments.

C. Activation of ASM as assessed by measuring the production of phosphorylcholine, a product of sphingomyelin hydrolation by ASM. A significant increase in phosphorylcholine production reflecting ASM activity was detected in cells after exposure to C. parvum for 15 min and 45 min.