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. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Immunol. 2008 Jan 15;180(2):1098–1106. doi: 10.4049/jimmunol.180.2.1098

Figure 4.

Figure 4

Chemokine/cytokine levels are elevated in the nervous system of chemokine knockout mice. WT, CXCL9−/− , CXCL10−/− mice (n=6/group) were infected with HSV-2 (2000 pfu/vagina). At indicated times, the mice were exsanguinated and the spinal cords and brain stems were removed, processed and assessed for CCL2, CCL3, CCL5, CXCL1 and TNF-α content using a suspension array system and ELISA. As there were no differences found in other analytes measured, only TNF-α level is shown for brain stem. Samples were analyzed in duplicate along with standard provided to generate standard curves for each analyte. The weight of the tissue was used to normalize amount of cytokine/chemokine per milligram of tissue weight. Day 0 time point represents uninfected controls. Each point represents the mean ± SEM summarizing the results of two independent experiments. *, p<.05 comparing the WT to CXCL9−/− and CXCL10−/−groups.