Figure 5.

T_c and I_Cl,swell in cells dialyzed with pipette solutions with altered osmolality and/or salt content. (A) Activation of I_Cl,swell (circles) without any increase in T_c (solid line) in a cell dialyzed with a low-salt pipette solution (Γ_p = 125 mM; 290 mOsm), further activation of I_Cl,swell by reducing OSM_o to 240 mOsm and inactivation of I_Cl,swell by increasing OSM_o to 400 mOsm. (B) Model prediction of V/V₀ (solid line) and Γ_i (broken line) for the experiment shown in A. (C) Increase in T_c (solid line) without significant activation of I_Cl,swell (circles) in a cell dialyzed with a high-salt pipette solution (Γ_p = 195 mM; 365 mOsm). Reducing OSM_o to 240 mOsm causes further cell swelling and activation of I_Cl,swell. (D) Same as B for the experiment shown in C. (E) Increase in T_c (solid line) and activation of I_Cl,swell (circles) in a cell dialyzed with a hypertonic pipette solution with normal salt content (Γ_p = 155 mM; 365 mOsm). (F) Same as B for the experiment shown in E.