Figure 6.

Application of negative pressure to the patch pipette does not affect activation of I_Cl,swell. (A) After break-in (time 0), a constant negative pressure was applied on the patch pipette, causing extensive shrinkage of the cell. Reducing OSM_o to 240 mOsm causes an increase of T_c (solid line) to levels that remain below the resting T_c (indicated by the broken line). Neither activation nor deactivation of I_Cl,swell (circles) is affected by the negative pressure. (B) Model prediction of V/V_o (solid line) and Γ_i (broken line) for the experiment shown in A.