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. Author manuscript; available in PMC: 2008 Jan 11.
Published in final edited form as: J Biol Chem. 2007 Nov 9;283(2):875–888. doi: 10.1074/jbc.M707907200

Figure 4. Kinetics of RNA synthesis using a luciferase-expressing, subgenomic replicon.

Figure 4

HeLa cells were transfected with the indicated replicon RNA (5 μg) and incubated for 4 h (A) or 8 h (B) post-transfection. Extracts were prepared and analyzed for luciferase activity. Luciferase specific activity is reported in relative light units (RLU) per microgram of total protein in the extract. WT + GuHCl represents a control for translation of input RNA without replication. The dashed line indicates luciferase activity observed for WT at 4 h. The results are the average of two independent transfections of replicon RNA transcribed from two independent clones. Error bars indicate the standard deviation.