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. Author manuscript; available in PMC: 2008 Jan 11.
Published in final edited form as: J Biol Chem. 2007 Nov 9;283(2):875–888. doi: 10.1074/jbc.M707907200

Figure 6. VPg uridylylation activity of selected PV 3C mutants.

Figure 6

A. Sypro stained gel of spin-column-purified proteins (0.1 μg) employed in the VPg uridylylation experiment. The sypro-stained proteins were detected by using a Typhoon-8600 Variable Mode Imager. B. VPg uridylylation reactions were performed by using the indicated purified 3C derivative. Uridylylation products were resolved from [α-32P]UTP by using a 15% polyacrylamide gel and a Tris-Tricine buffer system. The “-3C” lane indicates a reaction performed in the absence of 3C.