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. Author manuscript; available in PMC: 2008 Jan 11.
Published in final edited form as: J Biol Chem. 2007 Nov 9;283(2):875–888. doi: 10.1074/jbc.M707907200

Figure 7. OriI binding by selected PV 3C derivatives.

Figure 7

A. OriI binding by WT 3C. 3′-fluorescein-labeled oriI RNA (29-nt) was titrated with 3C. Binding was measured by monitoring the change in fluorescence polarization (Δ mP). The data were fit to a hyperbola. The Kd value is 260 ± 40 nM. B. OriI binding by 3C derivatives. 3C protein (150 nM) was mixed with 3′-fluorescein labeled oriI RNA and the change in polarization (Δ mP) was measured. The results are the average of three independent binding experiments with error bars indicating the standard deviation.