Table 2.
Diffusion coefficients (×1013 m2s−1) of the fluorescent fusion proteins expressed in plant protoplasts
| Construct(s) | D (CFP channel) | D (YFP channel) | D (Cross correlation channel) |
|---|---|---|---|
| AtSERK1-ECFP or -EYFP* | 4.0 ± 0.5 | 4.2 ± 0.6 | n.f. |
| AtSERK1-ECFP and -EYFP* | 4.2 ± 1.3 | 3.9 ± 0.7 | 2.6 ± 0.7 |
| AtSERK1ΔLZ-ECFP or -EYFP* | 3.7 ± 0.5 | 4.0 ± 0.5 | n.f. |
| AtSERK1ΔLZ-ECFP and -EYFP* | 4.2 ± 0.9 | 4.1 ± 0.6 | n.f. |
| AtSERK1kin-ECFP or -EYFP† | 90 ± 8 | 84 ± 5 | n.f. |
| AtSERK1kin-ECFP and -EYFP† | 78 ± 16 | 75 ± 15 | n.f. |
| AtSERK3-ECFP and -EYFP* | 4.4 ± 0.7 | 4.6 ± 0.6 | n.f. |
| BRI1-ECFP and -EYFP* | 4.5 ± 0.8 | 4.3 ± 0.5 | 3.8 ± 0.8 |
| CAAX-ECFP and -EYFP* | 5.3 ± 0.7 | 5.2 ± 0.7 | n.f. |
| CAAX-(ECFP)2* | 5.1 ± 0.8 | n.f. | n.f. |
| ECFP or EYFP† | 370 ± 41 | 410 ± 46 | n.f. |
The diffusion coefficients were obtained from FCS or FCCS analysis. All FCS curves were fitted according to a single-species model, except for the data of AtSERK1 and BRI1, which were fitted to a two-species model. The standard deviations represent the variation of the diffusion coefficient over experiments in different cells (n = 25–40 for each type of sample). n.f., not found.
*
Experiments in the plasma membrane.
†
Experiments in cytoplasmic regions lacking chloroplasts.