Table 1.
Physical properties of permeants
| Molecular weight (no counterion) | DAqueous (25°C) (10−6 cm2/s) | Stokes-Einstein radius (Å) | λex/λem (nM) | Net charge | |
|---|---|---|---|---|---|
| NBD-M-TMA | 280 | 5.8* | 4.3 | 458/580 | 1+ |
| Alexa 350 | 326 | 5.6* | 4.4 | 345/445 | 1− |
| Lucifer yellow | 443 | 5.0* | 4.9 | 428/536 | 2− |
| Alexa 488 | 546 | 4.2* | 5.8 | 493/517 | 2− |
| Alexa 594 | 734 | 3.3* | 7.4 | 588/613 | 2− |
| K | 39 | 19.6† | 1.3 | — | 1+ |
| Cl | 35 | 20.3† | 1.2 | — | 1− |
| TEA | 130 | 9.7† | 2.9 | — | 1− |
| Glutamate | 164 | 9.7‡ | 2.9 | — | 1+ |
*
Calculated using Wilke-Chang correlation (see Methods).
†
Calculated from mobility (32).
‡
Assumed equal to TEA due to similar solution conductivities.