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Number of donor and acceptor molecules and hence the minimum number of EGFRs per spot determined from the number of photobleaching steps in the associated donor and acceptor fluorescence versus time traces
| Minimum number of receptors | In pre-fixed cells | In cells stimulated with EGF |
|---|---|---|
| 2 | 7 | 12 |
| 3 | 7 | 9 |
| 4 | 1 | 4 |
| 5 | 1 | 2 |
