Table 3.

Photophysical and energetic parameters of PST-LOV proteins

	ΦT	E390/kJ/mol	Φ390	ΔVT/mL/mol	ΔV390/mL/mol
PST-LOVΔRR	0.58	144	0.51	−2.5	−2.0
PST-LOV	0.47	149	0.41	−2.9	−2.1
*PpSB2-LOV	0.46	133	0.42	−1.74	−16.0
†YtvA	0.62	136	0.49	−0.71	−12.5
†YtvA-LOV	0.69	113	0.55	−0.67	−17.2
‡Cr-phot-LOV1	0.63	171	0.6	−1.15	−8.0

The values of E_390, ΔV_T, and ΔV₃₉₀ are affected by the measurement error (<10%, see Table 2) and by the quantum yield determination, whose error depends on a variety of factors (e.g., absorption coefficients, reference standard values, and energy level of the triplet state). Repeated measurements (four) for Φ₃₉₀ gave an experimental variation within 8%.

^*Full-length SB2 LOV protein from Pseudomonas putida(35).

^†Full-length YtvA from Bacillus subtilis and its isolated LOV domain, YtvA-LOV (19).

^‡Isolated LOV1 domain of phototropin from Chlamydomonas reinhardtii(36).