Table 2.
Dissociation constants (Kd) expressed in terms of accessible acidic phosphospholipid (mean of two independent experiments ± SD) and measured by the cosedimentation assay using ezrin-Alexa488 or by fluorescence correlation spectroscopy
| ζ-potential (mV) | Kd (μM) Cosedimentation | Increase in Kd (fold) | Kd (μM) FCS | Increase in Kd (fold) | Cellular abundance (of all PI species) | |
|---|---|---|---|---|---|---|
| 20% PS | −27.7 ± 1.8 | 63.8 ± 10.5 | 16 | 580 ± 57 | 94 | – |
| PI(4,5)P2 | −24.5 ± 0.5 | 4.0 ± 0.5 | 1 | 6.2 ± 0.8 | 1 | ∼10%* |
| 5% PIP2 | NA | 4.2 ± 0.4 | 1.1 | 3.3 ± 0.8 | 0.5 | |
| 20% PS/5% PIP2 | NA | 4.1 ± 0.5 | 1.0 | NA | − | |
| 15% chol/5% PIP2 | ||||||
| 5% PI(4)P | −17.6 ± 1.6 | 13.1 ± 0.6 | 3.3 | 31.5 ± 9.4 | 5.1 | ∼10%* |
| 5% PI(3,4)P2 | −25.6 ± 1.2 | 5.3 ± 0.7 | 1.3 | 23.5 ± 5.6 | 3.8 | <0.15% |
| 5% PI(3,4,5)P3 | −30.5 ± 1.5 | 5.6 ± 0.7 | 1.4 | 9.6 ± 1.1 | 1.5 | <0.15% |
The ζ-potential of the LUVs is also given; NA, not available.
*
Values taken from Pendaries et al. (62).