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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Anal Biochem. 2007 Oct 11;373(1):167–169. doi: 10.1016/j.ab.2007.10.010

Fig. 2.

Fig. 2

Comparison of HO-1 activities from the heme titration and column chromatography methods – sHO-1 was quantified by two methods in order to validate the heme titration assay. The specific activities of sHO-1 from both methods were compared using a standard heme oxygenase assay, which uses the rate of bilirubin formation to determine activitHO-1 was added to a final concentration of 0.1 μM, with saturating levels of NADPH- cytochrome P450 reductase ranging from 0.2 μM to 0.5 μM. The specific activities of both samples were very similar, indicating that the heme titration can be used as an effective and accurate method of HO-1 quantitation.