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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Exp Neurol. 2007 Aug 28;208(2):207–215. doi: 10.1016/j.expneurol.2007.07.023

Figure 5.

Figure 5

Co-localization of ataxin-2 and ataxin-2 binding protein 1 (A1BP1) in transiently transfected COS1 cells. Single confocal sections through the nucleus are shown. COS1 cells were co-transfected with pDsRed2-A2BP1 (Red) and pEGFP-ataxin-2 (Green). After 24 hours, cells were immunofluorescently stained with mouse anti-Golgi58 antibody, and visualized with C5 conjugated antimouse IgG (Blue). A2BP1 colocalizes with full-length ataxin-2[Q22] in the Golgi apparatus. Expansion of the polyQ repeats disrupts the Golgi apparatus but ataxin-2[Q58] still colocalizes with A2BP1 in Golgi elements. Deletion within the LsmAD prevents ataxin-2 and A2BP1 localization in the Golgi apparatus, and retains A2BP1 in the nucleus.