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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Exp Neurol. 2007 Aug 28;208(2):207–215. doi: 10.1016/j.expneurol.2007.07.023

Figure 6.

Figure 6

Cytotoxicity of ataxin-2 by 7-aminoactinomycin D (7-AAD). Cells were transfected with full-length and truncated pEGFP-SCA2 containing different polyQ repeats. 24 hours posttransfection, cells were washed once with cold PBS, and incubated with 300 ul (~1 x 106 cells/ml) of PBS containing 1 ug/ml for 5 to 20 minutes on ice. Cells were then analyzed by flow cytometry. Percentage of cell death was determined by dividing the number of 7-AAD positive GFP expressing cells over the total number of GFP positive cells. Results were normalized with the average of cell death in cells expressing on the pEGFP plasmid (n =6). Relative cytotoxicity measured using the 7-AAD method and flow cytometry is similar to the trypan blue exclusion method. (One-way ANOVA, P<0.0001). The P values obtained from Tukey’s Multiple Comparison Test are shown above the respective bars.