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. Author manuscript; available in PMC: 2009 Jan 20.
Published in final edited form as: Virology. 2007 Nov 1;370(2):415–429. doi: 10.1016/j.virol.2007.09.001

Figure 9.

Figure 9

Inhibition of ssDNA hydrolysis by DBP. Two 70-μl reaction mixtures containing 3 μg/ml [3H]ssDNA E. coli, 25 mM Tris, pH 8.3, 50 mM KCl, 5 mM MgCl2, 100 μg/ml BSA, 12.5% glycerol, and 2 mM DTT were assembled on ice. DBP was added to one mixture to final concentration of 40 μg/ml, and both mixtures were incubated for 15 min at 30ºC. To initiate hydrolysis, 9 ng of the complex of AcMNPV alkaline nuclease with LEF-3 (complex AN/L3) was added to each reaction and incubation at 30ºC was continued. The 20-μl portions were taken from the mixtures at 10, 20, and 45 min and the release of acid-soluble radioactivity from [3H]ssDNA was measured. The radioactivity of undigested sample (5020 cpm) was taken as 100%.