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. 2007 Nov 12;7:209. doi: 10.1186/1471-2407-7-209

Figure 1.

Figure 1

Characterisation of inducible Smad4-reexpression in C4-II tet-on cell clones. a) Northern blot showing Smad4-specific mRNA in a number of independent clones derived from tet-on clones 18 and 28. Exposure of the left and middle panel was for one day, exposure of the right panel was five days. b) Time course of Smad4-induction in the "low-expressor" clone 18-2 and in the high-expressor clone 28-14 at the mRNA level. c) Time course of Smad4-induction in clones 18-2 and 28-14 at the protein level. d) Titration of Smad4-mRNA levels in clones 18-2 and 28-14 as compared to Smad4-reexpressing SW480 cell clones. The latter clones have previously been shown to express (sub-)physiological Smad4 levels. e) Titration of Smad4 protein levels in clones 18-2, 28-14 and 28-8.