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. 2007 Nov 12;7:209. doi: 10.1186/1471-2407-7-209

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Copyright © 2007 Klein-Scory et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Restoration of TGF-β responses in Smad4-reexpressing C4-II clones. a) Phosphorylation of R-Smad2/3 in response to TGF-β was assessed through Western blotting with a phospho-Smad2/3-specific antibody. b) TGF-β response in transient transfections with the p6SBE-luc promoter; the induction factor in Smad4-reexpressing clones is 5–8. c) TGF-β response in transient transfections with the p3TP-luc promoter; induction in Smad4-negative cells is approximately 1.5 and rises to 3–4 in Smad4-reexpressing clones.