Figure 2.

Electropherograms from capillary-based SDS/CGE on a six-protein mixture with an eCAP-coated capillary (a) and an uncoated fused-silica capillary (b). Capillary dimensions: 100-μm i.d. × 20 cm (27 cm in total length). Separation voltage: 8.1 kV. Injection: 4 kV for 30 sec. Peaks: (1) calmodulin (MM = 9 kDa); (2) α-lactalbumin (MM = 14.4 kDa); (3) pepsinogen (MM = 39 kDa); (4) egg albumin (MM = 45 kDa); (5) BSA (Mm = 66 kDa); and (6) β-galactosidase (MM = 116 kDa). Each protein concentration in the mixture was ≈1 × 10⁻⁹ M to 10 × 10⁻⁹ M. Injection: 4 kV for 30 s. (These conditions were optimized.)