Table 1.

Comparison of capillary-based vs. chip-based SDS/CGE for nonspecifically labeled proteins^a

^a Calculated from Figs. 2 and 3. All calculations were based on standard equations (27). 

^b For peaks 3 and 4, the resolution was calculated by using the expression R = 2 × (t₃ − t₄)/(Δt_1/2,3 + Δt_1/2,4), where t₃ and t₄ are the migration times of peaks 3 and 4 and Δt_1/2,3 and Δt_1/2,4 are the respective full widths at half maximum. 

^c Theoretical plate numbers (N) were calculated for peaks 2 and 5. These calculations were based on the measured full width at half maximum (Δt_1/2) of a peak by using the expression N = 5.54 × (t/Δt_1/2)², where t is the migration time. 

^d Plate heights (H) are calculated by dividing the column length by the theoretical plate numbers. For CE-based separations, a column length of 20 cm was used. For chip-based separations, a column length of 4.5 cm was used. 

^e Theoretical plates per second (N/s) were calculated by dividing theoretical plate numbers (N) by migration time.